Extraction Injection

PIC Solution’s proprietary Extraction-Injection (X-Injection, SFE-SFC) technique is available on our semi-preparative and preparative systems to help you purify poorly samples, speed-up isolation of minor impurities and separate sensitive compounds.

Extraction-Injection technique uses an extractor to dissolve the dry sample or biomass in the supercritical fluid prior to injection on the stationary phase. Our SFE-SFC technique is compatible with stacked injections and results in reproducible separations with excellent peak shapes. The quantity of sample injected can be tuned by changing time, temperature and pressure of extraction. Further, because the sample is dissolved in the mobile phase, the possibility of sample precipitation is eliminated.

Reference:

• Shaimi M. and Cox G.B., A Practical Comparison of Injection Procedures for Preparative SFC, Poster, SFC 2014, 2014 Basel, Switzerland.
• Shaimi M. and Cox G. B., Injection by Extraction: A Novel Sample Introduction Technique for Preparative SFC. Chromatography Today, 2014, 7, 4, 42
• Geoffrey B Cox, Injection by Extraction in Preparative SFC; Isolation of Impurities and Natural Products Chromatography Today, 2016, 9, 3, 16